LOCUS       40924_49322        3376 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Broad host range vector vector pCVD075, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, 
            Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Broad-host-range vector system for synthetic biology and 
            biotechnology in cyanobacteria
  JOURNAL   Nucleic Acids Res. (2014) In press
  PUBMED    25074377
REFERENCE   2  (bases 1 to 3376)
  AUTHORS   Taton A, Unglaub F, Wright N, Zeng WY, Paz Yepes J, Brahamsha B, 
            Palenik B, Peterson T, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2014) Division of Biological Sciences, University 
            of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA
REFERENCE   3  (bases 1 to 3376)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3376)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2014) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-JUN-2014) Division of Biological Sciences, University of 
            California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3376
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    370..375
                     /label=EcoRV
                     /note="EcoRV"
     misc_feature    complement(373..393)
                     /note="C2G; GC-adaptor for seamless assembly, flanking 
                     antibiotic resistance markers, functional devices or custom
                     sequences; synthetic"
     misc_feature    394..399
                     /label=AgeI
                     /note="AgeI"
     regulatory      400..571
                     /note="Perm; upstream region of erm ORF including promoter
                     and RBS; derived from pRL692 in INSD accession AF424805"
                     /regulatory_class="promoter"
     CDS             572..1303
                     /gene="ermC"
                     /label=ermC
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"
     regulatory      1339..1365
                     /note="trpA T; trpA terminator from Lorist6 INSD accession 
                     X98450, one nt shorter than reference; Lorist6"
                     /regulatory_class="terminator"
     misc_feature    1375..1380
                     /label=NheI
                     /note="NheI"
     misc_feature    complement(1381..1401)
                     /note="GC; GC-adaptor for seamless assembly, flanking
                     antibiotic resistance markers or Escherichia coli origins 
                     to be assembled with a cyanobacterial replicon; synthetic"
     misc_feature    1399..1404
                     /label=EcoRV
                     /note="EcoRV"
     rep_origin      complement(1629..2217)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2391..3248)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3249..3353)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattggcgg aaggccgtca
      361 aggccgcatg atatccggcg gcggcggcgg gataccggta ttcgatgaat tcgcgtgcta
      421 taattatact aattttataa ggaggaaaaa atatgggcat ttttagtatt tttgtaatca
      481 gcacagttca ttatcaacca aacaaaaaat aagtggttat aatgaatcgt taataagcaa
      541 aattcatata accaaattaa agagggttat aatgaacgag aaaaatataa aacacagtca
      601 aaactttatt acttcaaaac ataatataga taaaataatg acaaatataa gattaaatga
      661 acatgataat atctttgaaa tcggctcagg aaaaggccat tttacccttg aattagtaaa
      721 gaggtgtaat ttcgtaactg ccattgaaat agaccataaa ttatgcaaaa ctacagaaaa
      781 taaacttgtt gatcacgata atttccaagt tttaaacaag gatatattgc agtttaaatt
      841 tcctaaaaac caatcctata aaatatatgg taatatacct tataacataa gtacggatat
      901 aatacgcaaa attgtttttg atagtatagc taatgagatt tatttaatcg tggaatacgg
      961 gtttgctaaa agattattaa atacaaaacg ctcattggca ttacttttaa tggcagaagt
     1021 tgatatttct atattaagta tggttccaag agaatatttt catcctaaac ctaaagtgaa
     1081 tagctcactt atcagattaa gtagaaaaaa atcaagaata tcacacaaag ataaacaaaa
     1141 gtataattat ttcgttatga aatgggttaa caaagaatac aagaaaatat ttacaaaaaa
     1201 tcaatttaac aattccttaa aacatgcagg aattgacgat ttaaacaata ttagctttga
     1261 acaattctta tctcttttca atagctataa attatttaat aagtaagtta agggatgcag
     1321 gtcgactcta ggccggccag cccgcctaat gagcgggctt ttttttggcg cgccgctagc
     1381 atccgcgcgc gcgcgcgcga tatcctgggc ctcatgggcc ttccgctcac tgcccgcttt
     1441 ccagtcggga aacctgtcgt gccagctgca ttaacatggt catagctgtt tccttgcgta
     1501 ttgggcgctc tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc gggtaaagcc
     1561 tggggtgcct aatgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     1621 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     1681 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     1741 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     1801 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     1861 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     1921 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     1981 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     2041 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     2101 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     2161 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     2221 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     2281 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     2341 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     2401 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     2461 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     2521 ataccgcgag aaccacgctc accggctcca gatttatcag caataaacca gccagccgga
     2581 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     2641 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     2701 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     2761 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     2821 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     2881 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     2941 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     3001 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     3061 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     3121 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     3181 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     3241 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     3301 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     3361 ccccgaaaag tgccac
//
